Impedimetric Detection of microRNA at Graphene Oxide Modified Sensors

• Impedimetric miRNA detection was performed.
• Graphene oxide (GO) was modified onto the surface of graphite electrode (PGE)
• GO–PGE was characterized by microscopic and electrochemical techniques.

The graphene oxide (GO) modified pencil graphite electrodes (PGEs) were utilized for electrochemical monitoring of microRNA-34a (miRNA-34a) in this study. The GO concentration was firstly optimized after its modification onto the surface of disposable PGE by passive adsorption. The electrochemical behaviour of GO–PGEs was investigated by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) as well as microscopic characterization by using scanning electron microscopy (SEM). The impedimetric detection of hybridization between miRNA-34a target and its complementary DNA probe was recognized under the optimum conditions. The selectivity of the impedimetric genosensor was furtherly studied against to other miRNA sequences; such as; miRNA-15a, miRNA-155 and miRNA-660 in PBS (pH 7.40) or fetal bovine serum (FBS): PBS (1:1) diluted solution.

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