کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
185193 | 459592 | 2014 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Simultaneous Electroreduction of Different Diazonium Salts for Direct Electrochemical DNA Biosensor Development Simultaneous Electroreduction of Different Diazonium Salts for Direct Electrochemical DNA Biosensor Development](/preview/png/185193.png)
• Electroreduction of a mixture of diazonium salts.
• Combining surface spectroscopies (XPS and IRRAS)with electrochemistry to study mixed film.
• Bio-functionalization of mixed organic film for DNA electrochemical direct and signal-on biosensing.
• Optimizing the limit of detection to few pM of DNA target.
Mixed layers were obtained after one step deposition by reduction of a diazonium salts mixture. The layers are constituted of electroactive 5-hydroxy-1,4-naphthoquinone derivative (JUG), and 4-aminobenzoïc acid (ABA) which allows subsequent grafting of DNA probes. Each functionalizaton step was characterized by IRRAS and XPS. The redox properties of JUG were used to quantify this compound in the film. The combination of surface analysis and voltammetry leads to a quantitative characterization of all the components at each step. The resulting layers are composed of distinct JUG and ABA domains despite the one step deposition procedure. The developed approach allowed rationalization of experimental conditions to design direct electrochemical DNA biosensor with a detection limit of 10 pM.
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Journal: Electrochimica Acta - Volume 140, 10 September 2014, Pages 49–58