کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
18577 | 42726 | 2006 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: PVA-gel (Lentikats®) as an effective matrix for yeast strain immobilization aimed at heterologous protein production PVA-gel (Lentikats®) as an effective matrix for yeast strain immobilization aimed at heterologous protein production](/preview/png/18577.png)
Three different yeast strains, namely Saccharomyces cerevisiae mnn1mnn9, Kluyveromyces lactis JA6/GAA and Zygosaccharomyces bailii [pZ3klIL-1β], were entrapped in polyvinyl alcohol (PVA) gel particles, obtained following the commercially available immobilization kit named Lentikat®. After immobilization in the PVA-gel particles, yeast cells remained viable: colonization of the gel matrix reached up 100 mg d.w. of cells cm−3 gel for all the strains examined.Lentikats® of K. lactis JA6/GAA and Z. bailii [pZ3klIL-1β] showed to be suitable for the continuous production of glucoamylase and interleukin 1β, respectively, when employed under non-selective conditions. They were of easy handiness and showed excellent mechanical properties during prolonged operation in stirred tank reactors.
Journal: Enzyme and Microbial Technology - Volume 38, Issues 1–2, 3 January 2006, Pages 184–189