کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
185862 459604 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Large-scale synthesis of ultrathin Au-Pt nanowires assembled on thionine/graphene with high conductivity and sensitivity for electrochemical immunosensor
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Large-scale synthesis of ultrathin Au-Pt nanowires assembled on thionine/graphene with high conductivity and sensitivity for electrochemical immunosensor
چکیده انگلیسی

In this article, for the first time, a novel, label-free and inherent electroactive redox biosensor based on ultrathin Au-Pt nanowire-decorated thionine/reduced graphene oxide (AuPtNWs/THI/rGO) is developed for carcinoembryonic antigen (CEA) detection. Ultrathin AuPtNWs are prepared by a one-pot synthesis method without the use of any stabilizer or template. The AuPtNWs/THI/rGO composites are obtained by the THI/rGO composites surface functionalized with -NH2 group employed as a support for loading ultrathin AuPtNWs by coordination. The AuPtNWs/THI/rGO composites not only favor the immobilization of antibody but also facilitate the electron transfer. It is found that the resultant AuPtNWs/THI/rGO composites can be designed to act as a sensitive label-free electrochemical immunosensor for CEA determination. Under the optimized conditions, the linear range of the proposed immunosensor is estimated to be from 50 fg·mL−1 to 100 ng·mL−1 (R= 0.998) and the detection limit is estimated to be 6 fg·mL−1 at a signal-to-noise ratio of 3, respectively. The prepared immunosensor for detection of CEA shows high sensitivity, reproducibility and stability. Our study demonstrates that the proposed immunosensor has also been used to determine CEA successfully in diluted blood samples.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochimica Acta - Volume 130, 1 June 2014, Pages 335–343
نویسندگان
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