A review of experimental aspects of electrochemical immunosensors

In this brief review we want to give a guidance to all the researchers who want to challenge themselves in the task of developing electrochemical immunosensors for the first time. We will focus here only on practical aspects trying to give the readers useful insights that should be considered such as the choice of the electrode to be used, the best electrochemical and immunological procedures, the immobilization procedures, the instruments to purchase and other basic aspects of electrochemical immunosensors. In doing so we offer a wide view of the research on electrochemical immunosensor applications that have appeared in the literature in the last 5 years.

► Here we review practical aspects to develop electrochemical immunosensors.
► Wide view on the research on electrochemical immunosensors in the last 5 years.
► The review focuses on possible immobilization procedures and choice of electrodes.
► Low-cost and mass-production electrodes for immunosensors applications are discussed.
► Nanomaterials used to improve immunosensors performance are also reviewed.