کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
188560 | 459662 | 2012 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Development of an impedimetric DNA-biosensor based on layered double hydroxide for the detection of long ssDNA sequences Development of an impedimetric DNA-biosensor based on layered double hydroxide for the detection of long ssDNA sequences](/preview/png/188560.png)
DNA testing requires the development of sensitive and fast devices to measure the presence of nucleic acid sequences by DNA hybridization. In this paper, a simple and label-free DNA-biosensor has been investigated based on the detection of DNA hybridization on layered double hydroxide (LDH) nanomaterials with special emphasis on targeting long single stranded DNA sequences. First, the immobilization of a 20 bases long DNA probe on a thin layer of Mg2AlCO3 and Mg3AlCO3 LDH was studied. Then, DNA hybridization reaction was detected by means of Electrochemical Impedance Spectroscopy. The resulting biosensor showed a high sensitivity for the detection of 80 bases long DNA complementary sequences. The dynamic range was 18–270 ng/ml with a detection limit lower than 1.8 ng/ml.
Journal: Electrochimica Acta - Volume 74, 15 July 2012, Pages 123–129