Effects of sodium bis(2-ethylhexyl) sulfo succinate (AOT) reverse micelles on physicochemical properties of soy protein

• Physicochemical properties of soy proteins by different extraction methods were compared.
• Proteins from AOT reverse micelles had greater aggregates size and color difference.
• Surface hydrophobicity was greatly increased for proteins from AOT reverse micelles.
• The textural properties of the samples were different depending on their structure.
• Sulfhydryl/disulfide contents decreased for proteins from AOT reverse micelles.

The effects of AOT reverse micelle system on the physicochemical properties of soy proteins were studied in this work. The aggregate size of soy proteins using AOT reverse micelle extraction was much larger than that using aqueous buffer extraction. After AOT reverse micelle treatment, the color differences of protein powder are significative, sulfhydryl and disulfide contents of soy proteins decreased, but surface hydrophobicity greatly increased. Compared to soy proteins from aqueous buffer extraction, the enthalpy, onset temperature and peak temperature of the proteins from AOT reverse micelle extraction increased. The textural analyses of the gels of the soy proteins presented higher hardness, adhesiveness, gumminess, chewiness and lower cohesiveness, springiness.