کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
190337 459696 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Label-free impedimetric immunosensor for sensitive detection of atrazine
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Label-free impedimetric immunosensor for sensitive detection of atrazine
چکیده انگلیسی

Impedance spectroscopy transduction combined with the immunosensor technology has been used for the determination of atrazine, a herbicide The sensor electrode was based on the immobilization of anti-atrazine antibody by affinity binding onto a polypyrrole film N-substituted by nitrilotriacetic acid NTA electrogenerated on a gold electrode. The poly NTA film was previously modified by the coordination of Cu2 ions by the chelating NTA centers. The anti-atrazine antibody Fab fragment K47 modified with histidine-tag was then anchored by affinity interactions between the histidine-tag and the coordinated Cu2. Cyclic voltammetry experiments confirm that the antibody immobilization and the resulting immunosensor were applied to the impedimetric detection of atrazine without reagent and labelling step. The immunoreaction of atrazine on the attached anti-atrazine antibody directly triggers an increase in the charge transfer resistance proportional to the atrazine concentration, allowing the detection of extremely low atrazine concentration, namely 10 pg mL−1.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochimica Acta - Volume 55, Issue 21, 30 August 2010, Pages 6228–6232
نویسندگان
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