کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1905148 1534694 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Evaluation of urinary biomarkers of oxidative/nitrosative stress in adolescents and adults with Down syndrome
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
Evaluation of urinary biomarkers of oxidative/nitrosative stress in adolescents and adults with Down syndrome
چکیده انگلیسی

Urinary biomarkers of oxidative stress have been little studied in adults with Down syndrome (DS), usually no more than two biomarkers have been measured in the population studied and controversial results are reported in literature. Thus, we aimed to assess a set of oxidative and nitrosative stress biomarkers in urine samples of adolescents and adults with DS, with and without hypothyroidism, which comprise: 8-hydroxy-2′-deoxyguanosine (8-OHdG), isoprostane 15-F2t-IsoP, thiobarbituric acid-reacting substances (TBARS), advanced glycation end products (AGEs), dityrosine (diTyr), hydrogen peroxide (H2O2) and nitrite/nitrate (NOx). Fluorimetric and spectrophotometric assays were performed in DS (n = 78), some of them taking levothyroxine for hypothyroidism (n = 24), and in their healthy age-matched controls (n = 65). We found that levels of AGEs, diTyr, H2O2 and NOx are increased in DS patients in any or in all age groups, whereas Cr levels were lower in DS than in controls in all age groups. Besides, correlations with age in DS were positive for diTyr and negative for Cr, TBARS, 15-F2t-IsoP and NOx. We also found lower levels of Cr from 15 to 19 years, higher levels of TBARS and AGEs from 20 to 40 years and higher levels of diTyr from 15 to 40 years in DS patients receiving levothyroxine than in DS without hypothyroidism diagnosed. We conclude that AGEs, diTyr, H2O2 and NOx could be used as oxidative stress biomarkers in DS in contrast to 8-OHdG, 15-F2t-IsoP and TBARS, at least with the methods used. However, renal impairment could occur in DS and Cr adjustment may bias the results, particularly in hypothyroid patients.

Research highlights
► Urinary AGEs, diTyr, H2O2 and NOx could be used as oxidative stress biomarkers in DS.
► Urinary levels of 8-OHdG, 15-F2t-IsoP and TBARS are not altered in DS.
► Correlations with age were positive for diTyr and negative for Cr, TBARS, 15-F2t-IsoP and NOx in DS.
► Renal impairment could occur in DS and Cr adjustment may bias the results.
► Renal impairment due to hypothyroidism may bias the results in DS.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease - Volume 1812, Issue 7, July 2011, Pages 760–768
نویسندگان
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