کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1906000 1534754 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cysteine cathepsins in human silicotic bronchoalveolar lavage fluids
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
Cysteine cathepsins in human silicotic bronchoalveolar lavage fluids
چکیده انگلیسی

Mature, active cysteine cathepsins (CPs) were identified in human inflammatory bronchoalveolar lavage fluid (BALF) supernatants from patients suffering from silicosis by both western blot and surface plasmon resonance analyses. BALFs are not a reservoir of activatable proforms, since no autocatalytic maturation at acidic pH occurs. Cathepsin H is the most profuse among studied CPs (median value: 36.5 nM), while cathepsins B and L are the two most abundant thiol-dependent endoproteases. The overall concentration of active cathepsins B, H, K, L, and S is ∼10-fold lower than their concentration in BALF supernatants from patients suffering from inflammatory acute lung injuries (962 ± 347 nM).The cathepsins (approximately 70 nM)/cystatin-like inhibitors (approximately 9 nM) ratio is unbalanced in favor of enzymes (∼8-fold). This presence of uncontrolled CPs suggests that they may contribute, in addition to matrix metalloproteases, to the lung tissue breakdown/remodeling occurring during silicosis, although their exact contribution to interstitial inflammation remains to be evaluated.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease - Volume 1762, Issue 3, March 2006, Pages 351–356
نویسندگان
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