کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1909142 | 1046709 | 2011 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
An integrated approach to assessing nitroso-redox balance in systemic inflammation
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
RSNOGSSGGSHN-ethylmaleimideDNPHNOSS-nitrosothiolPaO2DCIPN-Nitroso compoundRNNOLPSTNFPBS2,4-dinitrophenyl - 2،4-dinitrophenyl2,6-dichlorophenolindophenol - 2،6-dichlorophenolindophenolROS - ROSEDTA - اتیلن دی آمین تترا استیک اسید Ethylenediaminetetraacetic acid - اتیلینیدامین تتراستیک اسیدDehydroascorbic acid - اسید DehydroascorbicEndotoxin - اندوتوکسینinterleukin - اینترلوکینintensive care unit - بخش مراقبتهای ویژهICU - بخش مراقبتهای ویژهDHA - دوکوساهگزائنوئیک اسیدFree radicals - رادیکال آزادSepsis - سپتیسمی یا مسمومیت خونtumor necrosis factor - فاکتور نکروز تومورarterial partial pressure of oxygen - فشار جزئی اکسیژن شریانیlipopolysaccharide - لیپوپلی ساکاریدPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریNEM - نهNitrosylation - نیتروسیلهNitrosation - نیتروژنNitrite - نیتریتNitric oxide - نیتریک اکسیدnitric oxide synthase - نیتریک اکسید سنتازglutathione (oxidized) - گلوتاتیون (اکسید شده)Glutathione (reduced) - گلوتاتیون (کاهش یافته)Reactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: An integrated approach to assessing nitroso-redox balance in systemic inflammation An integrated approach to assessing nitroso-redox balance in systemic inflammation](/preview/png/1909142.png)
چکیده انگلیسی
Most studies examining the metabolic fate of NO during systemic inflammation have focused on measuring the quantitatively predominating, stable anions nitrite and nitrate within the circulation. However, these are not necessarily the NO-related products that govern NO metabolism and signaling in tissues. We assessed all major NO derivatives temporally in blood and vital organs during inflammation and explored their relationship to insult severity and redox status. Male rats receiving intraperitoneal endotoxin or vehicle were sacrificed for organ and blood sampling between 0 and 24Â h. Endotoxin induced transient and organ-specific changes in a variety of NO metabolites. Nitrite and nitrate increased, peaking at 8 and 12Â h, respectively. S- and N-nitrosation and heme-nitrosylation products also peaked at 8Â h; these posttranslational protein modifications were associated with decreased myocardial function (echocardiography). Evidence of oxidative stress and systemic inflammation was also obtained. The rise in most NO derivatives was proportional to insult severity. All metabolite levels normalized within 24Â h, despite evidence of persisting myocardial dysfunction and clinical unwellness. Our findings point to a complex interplay between NO production, antioxidant defense, and redox status. Although the precise (patho)physiologic roles of specific NO derivatives and their diagnostic/prognostic utility await further investigation, nitroso species in erythrocytes are the most sensitive markers of NO in systemic inflammation, detectable before clinical symptoms manifest.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 51, Issue 6, 15 September 2011, Pages 1137-1145
Journal: Free Radical Biology and Medicine - Volume 51, Issue 6, 15 September 2011, Pages 1137-1145
نویسندگان
Alex Dyson, Nathan S. Bryan, Bernadette O. Fernandez, Maria-Francisca Garcia-Saura, Fumito Saijo, Nicolas Mongardon, Juan Rodriguez, Mervyn Singer, Martin Feelisch,