کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1909638 | 1046734 | 2009 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Lipoprotein lipase is nitrated in vivo after lipopolysaccharide challenge
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کلمات کلیدی
LPSiNOSRNSLPL2DE - 2deTwo-dimensional electrophoresis - الکتروفورز دو بعدیEndotoxin - اندوتوکسینinducible nitric oxide synthase - سنتاز اکسید نیتریک القاییTargeted mass spectrometry - طیف سنج جرمی هدفمندMass spectrometry - طیف سنجی جرمیLipoprotein lipase - لیپو پروتئین لیپازlipopolysaccharide - لیپوپلی ساکاریدnitrotyrosine - نیتروتیروستینreactive nitrogen species - گونه های واکنش پذیر نیتروژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Lipopolysaccharide (LPS) administration down-regulates lipoprotein lipase (LPL) activity at the posttranscriptional level. Hypertriglyceridemia is the main metabolic consequence of this fall in LPL activity and is presumably involved in the innate immune response to infection. Nitric oxide (NO) has been implicated in LPS-induced down-regulation of LPL activity, but whether its effects are direct or indirect remains unclear. Here we examined the potential nitration of LPL in vivo in response to LPS challenge in rats. We found hypertriglyceridemia, iNOS expression, NO overproduction, and a generalized decrease in LPL activity in tissues 6Â h after LPS administration. LPL sensitivity to nitration was first explored by in vitro exposure of bovine LPL to peroxynitrite, a reactive nitrogen species (RNS). Nitration was confirmed by anti-nitrotyrosine Western blot and subsequent identification of specific nitrotyrosine-containing LPL sequences by tandem mass spectrometry. Further analysis by targeted mass spectrometry revealed three in vivo-nitrated tyrosine residues in heart LPL from LPS-challenged rats. This is the first study to identify nitrated tyrosine residues in LPL, both in vitro and in vivo, and it demonstrates that LPL is a target for RNS in endotoxemia. These results indicate that LPL nitration may be a new mechanism of LPL activity regulation in vivo.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 47, Issue 11, 1 December 2009, Pages 1553-1560
Journal: Free Radical Biology and Medicine - Volume 47, Issue 11, 1 December 2009, Pages 1553-1560
نویسندگان
Albert Casanovas, Montserrat Carrascal, JoaquÃn Abián, M. Dolores López-Tejero, Miquel Llobera,