کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1911337 | 1046812 | 2006 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Intrinsic disturbance of cellular redox balance enhances blood lymphocyte apoptosis in the spontaneously hypertensive rat
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کلمات کلیدی
SSCTNFWKYNADPHFSCPBMCROS - ROSOxidative stress - تنش اکسیداتیوApoptosis - خزان یاختهایFree radicals - رادیکال آزادperipheral blood mononuclear cells - سلول های تک هسته ای خون محیطیShr - شریTissue necrosis factor - عامل نکروز بافتHypertension - فشار خون بالاLymphocyte - لنفوسیتHydroethidine - هیدروتیدینWistar-Kyoto - ویستار کیوتوforward scatter - پراکندگی رو به جلوside scatter - پراکندگی سمتreduced nicotinamide adenine dinucleotide phosphate - کاهش نیکوتین آمید آدنین دینکلوتید فسفاتReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Intrinsic disturbance of cellular redox balance enhances blood lymphocyte apoptosis in the spontaneously hypertensive rat Intrinsic disturbance of cellular redox balance enhances blood lymphocyte apoptosis in the spontaneously hypertensive rat](/preview/png/1911337.png)
چکیده انگلیسی
The spontaneously hypertensive rat (SHR) shows an altered cell apoptosis rate compared to normotensive controls by a mechanism that may involve redox imbalance. This study was designed to determine whether cellular oxidative stress and apoptosis in blood lymphocytes are enhanced in the SHR by intrinsic cellular abnormalities and/or by factors derived from selected organs (kidney, adrenals). We identified apoptosis and free radical production in isolated peripheral blood lymphocytes with flow cytometry before and after serum withdrawal, which exposes the cells to a pro-oxidative condition. Freshly drawn SHR lymphocytes showed higher levels of apoptosis and intracellular oxygen radicals than lymphocytes from normotensive Wistar-Kyoto rats (p < 0.05). Apoptosis and intracellular oxygen radicals were profoundly elevated after serum-free incubation in SHR cells and to a lesser extent in normotensive controls suspended in exactly the same medium (p < 0.01, SHR vs normotensives). Cell-permeable antioxidants, l-cysteine and Tempol, attenuated serum deprivation-induced apoptosis as well as cellular oxidative stress. Blood plasma and organ extracts from the SHR exerted pro- or antiapoptotic activity to the same degree as those from normotensives. The results suggest that blood lymphocyte apoptosis is enhanced in the SHR due to intrinsically disturbed cellular redox balance rather than altered activity in extracellular humoral factors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 41, Issue 3, 1 August 2006, Pages 484-492
Journal: Free Radical Biology and Medicine - Volume 41, Issue 3, 1 August 2006, Pages 484-492
نویسندگان
Nobuhiko Kobayashi, Geert W. Schmid-Schönbein,