کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1911389 | 1046815 | 2006 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Distribution of macular xanthophylls between domains in a model of photoreceptor outer segment membranes
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کلمات کلیدی
DSMRaft domainsPhotoreceptor outer segmentCSLAMDDSPCDRMDOPCdistearoylphosphatidylcholine - distearoyl فسفاتیدیل کولینsphingomyelin - اسفنگومیلینDHA - دوکوساهگزائنوئیک اسیدdioleoylphosphatidylcholine - دیئولیل فسفاتیدیل کولینFree radical - رادیکالهای آزاد Zeaxanthin - زاکسانتیینage-related macular degeneration - سن تخریب ماکولا مربوط به سن استRetina - شبکیهdetergent-resistant membrane - غشای مقاوم در برابر مواد شویندهphosphatidylcholine - فسفاتیدیل کولینphosphatidylethanolamine - فسفاتیدیلتانولامینAntioxidant activity - فعالیت آنتیاکسیدانیLutein - لوتئین، ویتامین چشمpos - پست
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A model of photoreceptor outer segment (POS) membranes has been proposed, consisting of an equimolar ternary mixture of 1-palmitoyl-2-docosahexaenoylphosphatidylcholine/distearoylphosphatidylcholine/cholesterol. It was shown that, as in membranes made from the raft-forming mixture, in the model of POS membranes, two domains are formed: the raft domain (detergent resistant membranes, DRM), and the bulk domain (detergent soluble membranes, DSM). Saturation-recovery EPR discrimination by oxygen transport method also demonstrated the presence of two domains in this model system in situ at a wide range of temperatures (10-55°C), showing additionally that neither lutein nor zeaxanthin at 1 mol% affect the formation of these domains. These membrane domains have been separated using cold Triton X-100 extraction from a model of POS membranes containing 1 mol% of either lutein or zeaxanthin. The results indicated that the macular xanthophylls lutein and zeaxanthin are substantially excluded from DRM and remain concentrated in DSM, a domain enriched in highly unsaturated docosahexaenoyl acid which is abundant in retina membranes. The concentration of xanthophylls in DRM and DSM calculated as the mol ratio of either xanthophyll to total lipid (phospholipid + cholesterol) was 0.0028 and 0.0391, respectively. Thus, xanthophylls are about 14 times more concentrated in DSM than in DRM. No significant difference in the distribution of lutein and zeaxanthin was found. The obtained results suggest that in POS membranes macular xanthophylls should also be concentrated in domains enriched in polyunsaturated chains.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 41, Issue 8, 15 October 2006, Pages 1257-1265
Journal: Free Radical Biology and Medicine - Volume 41, Issue 8, 15 October 2006, Pages 1257-1265
نویسندگان
Anna Wisniewska, Witold K. Subczynski,