کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1911774 | 1046832 | 2006 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Basal reactive oxygen species determine the susceptibility to apoptosis in cirrhotic hepatocytes
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کلمات کلیدی
2,3-dimethoxy-1,4-naphthoquinonemitotracker redDPPDH2-DCFDADMNQMTRNACTGFβMPT2′,7′-dichlorofluorescein diacetate - 2 '، 7'-dichlorofluorescein diacetateHepatocytes - hepatocytesN-acetylcysteine - N-استیل سیستئینROS - ROSmitochondrial permeability transition - انتقال نفوذپذیری میتوکندریTransforming Growth Factor Beta - تبدیل بتا فاکتور رشدApoptosis - خزان یاختهایMitochondria - میتوکندریاPropidium iodide - پروتئین یدیدReactive oxygen species (ROS) - گونه های اکسیژن واکنشی (ROS)Reactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
سالمندی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Hepatocytes from cirrhotic murine livers exhibit increased basal ROS activity and resistance to TGFβ-induced apoptosis, yet when ROS levels are decreased by antioxidant pretreatment, these cells recover susceptibility to apoptotic stimuli. To further study these redox events, hepatocytes from cirrhotic murine livers were pretreated with various antioxidants prior to TGFβ treatment and the ROS activity, apoptotic response, and mitochondrial ROS generation were assessed. In addition, normal hepatocytes were treated with low-dose H2O2 and ROS and apoptotic responses determined. Treatment of cirrhotic hepatocytes with various antioxidants decreased basal ROS and rendered them susceptible to apoptosis. Examination of normal hepatocytes by confocal microscopy demonstrated colocalization of ROS activity and respiring mitochondria. Basal assessment of cirrhotic hepatocytes showed nonfocal ROS activity that was abolished by antioxidants. After pretreatment with an adenovirus expressing MnSOD, basal cirrhotic hepatocyte ROS were decreased and TGFβ-induced colocalization of ROS and mitochondrial respiration was present. Treatment of normal hepatocytes with H2O2 resulted in a sustained increase in ROS and resistance to TGFβ apoptosis that was reversed when these cells were pretreated with an antioxidant. In conclusion, cirrhotic hepatocytes have a nonfocal distribution of ROS. However, normal and cirrhotic hepatocytes exhibit mitochondrial localization of ROS that is necessary for apoptosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Free Radical Biology and Medicine - Volume 41, Issue 11, 1 December 2006, Pages 1645-1654
Journal: Free Radical Biology and Medicine - Volume 41, Issue 11, 1 December 2006, Pages 1645-1654
نویسندگان
Jay Raval, Suzanne Lyman, Takashi Nitta, Dagmara Mohuczy, John J. Lemasters, Jae-Sung Kim, Kevin E. Behrns,