کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1919333 1535630 2012 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of aging and caloric restriction on brainstem satiety center signals in rats
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
Effects of aging and caloric restriction on brainstem satiety center signals in rats
چکیده انگلیسی

Age-related increases of body weight and adiposity, indicating dysregulation of food intake/energy expenditure, can be prevented in rodents by long-term 40% caloric restriction. The dorsal vagal complex (DVC), the brainstem center mediating the satiety reflex, has recently emerged as a determinant effector of long-term feeding adaptation. To study the effects of aging and caloric restriction on satiety circuits, leptin and brain-derived neurotrophic factor (BDNF) signaling systems were studied in 2- and 19-month-old ad libitum-fed (AL) and 19-month-old calorie-restricted (CR) rats. Age-induced hyperleptinemia in AL rats was correlated with elevated DVC BDNF immunoreactive concentrations and satiety threshold stability, suggesting functional desensitization of the DVC to these signals. To better understand this phenomenon, mRNA levels of receptor and post-receptor signaling effectors were measured by real-time RT-PCR. Aging selectively increased BDNF receptors and suppressor of cytokine signaling-3 (SOCS-3) mRNA levels. Caloric restriction prevented age-related increases of serum leptin, DVC BDNF and SOCS-3 mRNA levels, but not those of BDNF receptors. In CR rats, prevention of leptin resistance-promoting SOCS-3 induction was also observed at the protein level. This study suggests that leptin post-receptor targets and BDNF signaling play a role in the establishment of age-related DVC dysfunction.


► We studied ageing effects on satietogenic signaling in rat dorsal vagal complex (DVC).
► Rats aged with long-term caloric restriction (CR) versus ad libitum feeding (AL).
► Age-induced hyperleptinemia in AL rats was correlated with increased DVC BDNF.
► Ageing in AL rats increased DVC suppressor of cytokine signaling-3 (SOCS-3) mRNA.
► CR prevented age-induced rises in serum leptin, DVC BDNF and SOCS-3 mRNA and protein levels.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Mechanisms of Ageing and Development - Volume 133, Issues 2–3, February–March 2012, Pages 83–91
نویسندگان
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