Oxidation of organic and biogenic amines by recombinant human hephaestin expressed in Pichia pastoris

► Expression of the human hephaestin ectodomain in Pichia pastoris. ► Kinetic analysis of hephaestin ferroxidase and amine oxidase activities. ► Iron as a mediator in the hephaestin-catalyzed oxidation of p-phenilenediamine. ► Inability of hephaestin to oxidize biogenic amines directly. ► Enzymatic difference between hephaestin and ceruloplasmin.