|کد مقاله||کد نشریه||سال انتشار||مقاله انگلیسی||ترجمه فارسی||نسخه تمام متن|
|1926193||1536444||2010||11 صفحه PDF||سفارش دهید||دانلود رایگان|
Flavoprotein monooxygenases reduce flavins, speed their reaction with oxygen, and stabilize a C4a-oxygen adduct long enough to use this reactive species to transfer an oxygen atom to a substrate. The flavin–oxygen adduct can be the C4a-peroxide anion, in which case it reacts as a nucleophile. The protonated adduct – the C4a-hydroperoxide – reacts as an electrophile. The elimination of H2O2 competes with substrate oxygenation. This side-reaction is suppressed, preventing the waste of NAD(P)H and the production of toxic H2O2. Several strategies have been uncovered that prevent the deleterious side-reaction while still allowing substrate hydroxylation.
Journal: Archives of Biochemistry and Biophysics - Volume 493, Issue 1, 1 January 2010, Pages 26–36