کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1926280 | 1536449 | 2009 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Kinetic analysis provides insight into the mechanism of Ribonuclease A oligomer formation
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Ribonuclease A forms a series of oligomers by 3D domain swapping, a possible mechanism for amyloid formation. Using experimental data, the Ribonuclease oligomerization process is analyzed to obtain estimates of individual equilibrium and microscopic rate constants. The results suggest several novel insights into Ribonuclease oligomer formation: (i) two dimers may combine to yield tetramers, (ii) the lower abundance of the cyclic trimer could be ascribed to the cis conformation of its Asn113-Pro114 peptide bonds, (iii) oligomers become the dominant species at very high protein concentrations or upon applying a modest tenfold increase in the equilibrium constants (iv) the rate constants for trimer and tetramer formation are faster than those of dimer formation and (v) glycosylation affects the relative populations of different trimer and tetramer species. By mass spectrometry, oligomers as large as tetradecamers are detected. These results are consistent with the proposal that 3D domain swapping is a mechanism for amyloid formation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 489, Issues 1â2, September 2009, Pages 41-47
Journal: Archives of Biochemistry and Biophysics - Volume 489, Issues 1â2, September 2009, Pages 41-47
نویسندگان
Jorge P. López-Alonso, Giovanni Gotte, Douglas V. Laurents,