کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1927233 1536506 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Binding of bovine prion protein to heparin: A fluorescence polarization study
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Binding of bovine prion protein to heparin: A fluorescence polarization study
چکیده انگلیسی

Glycosaminoglycans (GAGs) are believed to be associated with prion disease pathology and also with metabolism of the prion protein. Fluorescence polarization assay (FPA) of binding between bovine recombinant prion protein (brecPrP) and heparin labelled with AlexaFluor488 was used in model experiments to study glycosaminoglycan–prion protein interaction. Heparin binding to brecPrP was a rapid reversible event which occurred under defined conditions. The interaction of brecPrP with fluorophore-labelled heparin was inhibited by the presence of Cu2+ ions and was sensitive to competition with heparin, heparan sulphate, and dextran. The dissociation constant of the heparin–brecPrP complex was 73.4 ± 3.7 nM. Circular dichroism (CD) experiments indicated that the structure of brecPrP was less helical in the presence of heparin.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Archives of Biochemistry and Biophysics - Volume 460, Issue 1, 1 April 2007, Pages 10–16
نویسندگان
, , , ,