کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
192780 | 459751 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A novel DNA biosensor based on ssDNA/Cyt c/l-Cys/GNPs/Chits/GCE
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
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چکیده انگلیسی
A novel DNA biosensor was fabricated by modified multilayer of ssDNA, cytochrome c, l-cysteine, metal gold nanoparticles and Chitosan (denoted as ssDNA/Cyt c/l-Cys/GNPs/Chits/GCE). The behavior of the DNA biosensor was then investigated by voltammetry, impedance spectrum and atomic force microscope (AFM), and the morphologic differences among each layer of the DNA biosensor were also observed. Results revealed that two well-defined redox peaks exhibited at 0.120 V and 0.362 V, and the amount of adsorbed DNA was 1.672 × 10−10 mol cm−2. We concluded that the modified electrode could be used to detect DNA with the indicator daunomycin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochimica Acta - Volume 54, Issue 8, 1 March 2009, Pages 2284–2289
Journal: Electrochimica Acta - Volume 54, Issue 8, 1 March 2009, Pages 2284–2289
نویسندگان
Mengyao Li, Shasheng Huang, Peisi Zhu, Lingmi Kong, Bin Peng, Huan Gao,