کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1927957 | 1050299 | 2015 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: MicroRNA-761 regulates mitochondrial biogenesis in mouse skeletal muscle in response to exercise MicroRNA-761 regulates mitochondrial biogenesis in mouse skeletal muscle in response to exercise](/preview/png/1927957.png)
• Endurance exercise decreases miR-761 expression in skeletal muscle.
• MiR-761 suppresses mitochondrial biogenesis in C2C12 myocytes.
• MiR-761 directly targeted PGC-1α expression.
• MiR-761 suppresses p38 MAPK signaling pathways in C2C12 myocytes.
• A novel mechanism for miR-761 in skeletal myocytes is demonstrated.
MicroRNAs (miRNAs) have been suggested to play critical roles in skeletal muscle in response to exercise. Previous study has shown that miR-761 was involved in a novel model regulating the mitochondrial network. However, its role in mitochondrial biogenesis remains poorly understood. Therefore, the current study was aimed to examine the effect of miR-761 on mitochondrial biogenesis in skeletal muscle. Real-time quantitative PCR analysis demonstrated that aberrantly expressed miR-761 is involved in exercise activity and miR-761 is decreased by exercise training compared with the sedentary control mice. miR-761 suppresses mitochondrial biogenesis of C2C12 myocytes by targeting the 3′-UTR of peroxisome proliferator-activated receptor gamma (PPARγ) coactivator-1 (PGC-1α). Overexpression of miR-761 was capable of inhibiting the protein expression levels of PGC-1α. Moreover, miR-761 overexpression suppressed the p38 MAPK signaling pathway and down-regulated the expression of phosphorylated MAPK-activated protein kinase-2 (P-MK2), a downstream kinase of p38 MAPK. The phosphorylation of activating transcription factors 2 (ATF2) that plays a functional role in linking the activation of the p38 MAPK pathway to enhanced transcription of the PGC-1α was also inhibited by the overexpression of miR-761. These findings revealed a novel regulation mechanism for miR-761 in skeletal myocytes, and contributed to a better understanding of the modulation of skeletal muscle in response to exercise.
Journal: Biochemical and Biophysical Research Communications - Volume 467, Issue 1, 6 November 2015, Pages 103–108