کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1928093 1050312 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Ovary-dependent emphysema augmentation and osteopontin induction in adult female mice
ترجمه فارسی عنوان
القاء آمفیزم وابسته به تخمدان و القاء استئوپونتیک در موش های ماده بالغ
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


• Adult female mice show more severe, elastase-induced emphysema than adult male mice.
• Ovaries enhance elastase-induced lung inflammation and alveolar destruction.
• Ovary-dependent osteopontin induction may contribute to emphysema augmentation.

Biological differences between the sexes greatly impact the development and severity of pulmonary disorders such as emphysema. Recent studies have demonstrated crucial roles for osteopontin (OPN, also known as SPP1) in lung inflammation and alveolar destruction in human and experimental emphysema, but the impact of gender on OPN action remains unknown. Here, we report ovary-dependent induction of Opn mRNA with augmentation of experimental emphysema in adult female mice. Both male and female mice developed emphysematous lungs following intra-tracheal administration of porcine pancreatic elastase; however, compared with male mice, female mice developed more severe injury-related inflammation and pathologic alterations of the lungs. Notably, we observed female-specific induction of the Opn gene upon lung injury. Ovariectomy blocked this induction, with attenuation of lung inflammation and alveolar destruction, demonstrating the essential role of ovaries in injury-related Opn induction and augmentation of emphysema in adult female mice. Lastly, pre-treatment of adult female mice with pyridoxalphosphate-6-azophenyl-2′,4′-disulfonic acid, which blocks ATP-mediated wound response, suppressed Opn mRNA induction upon lung injury, resulting in attenuation of enhanced lung inflammation. Together, our findings define a novel, ovary-dependent mechanism underlying gender-specific augmentation of emphysema through transcriptional control of the Opn gene.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 461, Issue 4, 12 June 2015, Pages 642–647
نویسندگان
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