H2S does not regulate proliferation via T-type Ca2+ channels

• T-type Ca2+ channels regulate proliferation and are sensitive to the gasotransmitters CO and H2S.
• H2S reduced proliferation in HEK293 cells expressing the H2S sensitive Cav3.2 channel.
• H2S also inhibited proliferation in non-transfected cells and HEK293 cells expressing Cav3.1.
• Native smooth muscle cells primarily express Cav3.1. Their proliferation was also inhibited by H2S.
• Unlike CO, H2S does not regulate smooth muscle proliferation via T-type Ca2+ channel inhibition.

T-type Ca2+ channels (Cav3.1, 3.2 and 3.3) strongly influence proliferation of various cell types, including vascular smooth muscle cells (VSMCs) and certain cancers. We have recently shown that the gasotransmitter carbon monoxide (CO) inhibits T-type Ca2+ channels and, in so doing, attenuates proliferation of VSMC. We have also shown that the T-type Ca2+ channel Cav3.2 is selectively inhibited by hydrogen sulfide (H2S) whilst the other channel isoforms (Cav3.1 and Cav3.3) are unaffected. Here, we explored whether inhibition of Cav3.2 by H2S could account for the anti-proliferative effects of this gasotransmitter. H2S suppressed proliferation in HEK293 cells expressing Cav3.2, as predicted by our previous observations. However, H2S was similarly effective in suppressing proliferation in wild type (non-transfected) HEK293 cells and those expressing the H2S insensitive channel, Cav3.1. Further studies demonstrated that T-type Ca2+ channels in the smooth muscle cell line A7r5 and in human coronary VSMCs strongly influenced proliferation. In both cell types, H2S caused a concentration-dependent inhibition of proliferation, yet by far the dominant T-type Ca2+ channel isoform was the H2S-insensitive channel, Cav3.1. Our data indicate that inhibition of T-type Ca2+ channel-mediated proliferation by H2S is independent of the channels’ sensitivity to H2S.