کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1928170 | 1536772 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Na+-Ca2+ exchanger targeting miR-132 prevents apoptosis of cardiomyocytes under hypoxic condition by suppressing Ca2+ overload
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Na+-Ca2+ exchanger targeting miR-132 prevents apoptosis of cardiomyocytes under hypoxic condition by suppressing Ca2+ overload Na+-Ca2+ exchanger targeting miR-132 prevents apoptosis of cardiomyocytes under hypoxic condition by suppressing Ca2+ overload](/preview/png/1928170.png)
چکیده انگلیسی
During ischemia-reperfusion (IR) injury of the heart, Ca2+ overload occurs, leading to cardiomyocyte dysfunction and eventual cell death by apoptosis. Since preventing Ca2+ overload during IR injury has been reported to protect cardiomyocytes, interrupting Ca2+ signaling cascades leading to Ca2+ overload may exert protective effect on cardiomyocytes under hypoxic condition. One of the key regulators of the intracellular Ca2+ level during IR injury is Na+-Ca2+ exchanger 1 (NCX1), whose down-regulation during IR injury conferred protection of heart. In the present study, we examined whether down-regulation of NCX1 using exogenous microRNA ameliorates apoptosis of cardiomyocytes under hypoxic condition. Here, we identified miR-132 as a novel microRNA targeting the NCX1, whose expression increased during hypoxia. Delivery of miR-132 suppressed the increase of intracellular Ca2+ in cardiomyocytes under hypoxia, and the expressions of apoptotic molecules, such as Bax, cytochrome C, and caspase 3, and the number of apoptotic cells were also decreased by exogenous miR-132 treatment. These results suggest the potential of miR-132 as an effective therapeutic agent against IR damage to heart by preventing Ca2+ overload during hypoxic condition and warrant further studies to validate its anti-apoptotic effect in vivo.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 460, Issue 4, 15 May 2015, Pages 931-937
Journal: Biochemical and Biophysical Research Communications - Volume 460, Issue 4, 15 May 2015, Pages 931-937
نویسندگان
Soonchang Hong, Jiyun Lee, Hyang-Hee Seo, Chang Yeon Lee, Kyung-Jong Yoo, Sung-Man Kim, Seahyoung Lee, Ki-Chul Hwang, Eunhyun Choi,