کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1928501 1050363 2014 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Allograft inflammatory factor-1 stimulates chemokine production and induces chemotaxis in human peripheral blood mononuclear cells
ترجمه فارسی عنوان
عامل التهاب آلوگرافت 1 باعث تولید کیموکین می شود و شیمی درمانی را در سلول های تک هسته ای خون محیطی انسان ایجاد می کند
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


• rhAIF-1 stimulation upregulated chemokine gene expressions in CD14+ PBMCs.
• rhAIF-1 promoted the secretion of CCL3/MIP-1α and IL-6 by CD14+ PBMCs.
• Conditioned media from rhAIF-1stimulated CD14+ PBMCs induced migration of PBMCs.
• AIF-1 may be a molecular target for the therapy of immune-inflammatory disorders.

Allograft inflammatory factor-1 (AIF-1) is expressed by macrophages, fibroblasts, endothelial cells and smooth muscle cells in immune-inflammatory disorders such as systemic sclerosis, rheumatoid arthritis and several vasculopathies. However, its molecular function is not fully understood. In this study, we examined gene expression profiles and induction of chemokines in monocytes treated with recombinant human AIF (rhAIF-1). Using the high-density oligonucleotide microarray technique, we compared mRNA expression profiles of rhAIF-1-stimulated CD14+ peripheral blood mononuclear cells (CD14+ PBMCs) derived from healthy volunteers. We demonstrated upregulation of genes for several CC chemokines such as CCL1, CCL2, CCL3, CCL7, and CCL20. Next, using ELISAs, we confirmed that rhAIF-1 promoted the secretion of CCL3/MIP-1α and IL-6 by CD14+ PBMCs, whereas only small amounts of CCL1, CCL2/MCP-1, CCL7/MCP-3 and CCL20/MIP-3α were secreted. Conditioned media from rhAIF-1stimulated CD14+ PBMCs resulted in migration of PBMCs. These findings suggest that AIF-1, which induced chemokines and enhanced chemotaxis of monocytes, may represent a molecular target for the therapy of immune-inflammatory disorders.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 448, Issue 3, 6 June 2014, Pages 287–291
نویسندگان
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