Identification of a novel Pfkfb1 mRNA variant in rat fetal liver

The bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase-2) catalyzes the synthesis and degradation of fructose-2,6-bisphosphate, a key metabolite in the glucose homeostasis. Four genes, Pfkfb1–4, have been characterized in mammals that code for several isoforms generated by alternative splicing through the control of several promoters and 5′ non-coding exons. Here, we characterize in fetal rat liver new mRNA variants which are transcribed from a new Pfkfb1 gene promoter. The long variant codes to a new isoform (FL-PFK-2) that would be of relevant function to modulate the transition of fetal to adult liver metabolism.

► We have identified a novel Pfkfb1 mRNA variant in rat fetal liver.
► FL-PFK-2 variants are transcribed from a new Pfkfb1 promoter.
► FL-long variant encoded a functional protein with kinase activity.