کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1929290 1050451 2012 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Drought-induced activation and rehydration-induced inactivation of MPK6 in Arabidopsis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Drought-induced activation and rehydration-induced inactivation of MPK6 in Arabidopsis
چکیده انگلیسی

Mitogen-activated protein kinases (MPKs) have roles in regulating developmental processes and responses to various stimuli in plants. Activations of some MPKs are necessary for proper responses to hyperosmolarity and to a stress-related phytohormone, abscisic acid (ABA). However, there is no direct evidence that MPK activations are regulated by drought and rehydration. Here we show that the activation state of one of the Arabidopsis MPKs, MPK6, is directly regulated by drought and rehydration. An immunoblot analysis using an anti-active MPK antibody detected drought-induced activation and rehydration-induced inactivation of MPK6. MPK6 was activated by drought even in an ABA-deficient mutant, aba2-4. In addition, exogenously added ABA failed to suppress the rehydration-dependent inactivation of MPK6. Under drought conditions, elevated levels of reactive oxygen species (ROS), which are known elicitors of MPK6 activation, were detected in both wild type and an MPK6-deficient mutant, mpk6-4. These results suggest that ROS, but not ABA, induces MPK6 activation as an upstream signal under drought conditions.


► Arabidopsis MPK6 is activated by drought and inactivated by rehydration.
► Drought-induced activation of MPK6 is independent of abscisic acid.
► Drought-induced production of reactive oxygen species probably activates MPK6.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 426, Issue 4, 5 October 2012, Pages 626–629
نویسندگان
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