کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1929370 | 1050453 | 2012 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Homer proteins accelerate Ca2+ clearance mediated by the plasma membrane Ca2+ pump in hippocampal neurons Homer proteins accelerate Ca2+ clearance mediated by the plasma membrane Ca2+ pump in hippocampal neurons](/preview/png/1929370.png)
The plasma membrane Ca2+ ATPase (PMCA) is responsible for maintaining basal intracellular Ca2+ concentration ([Ca2+]i) and returning small increases in [Ca2+]i back to resting levels. The carboxyl terminus of some PMCA splice variants bind Homer proteins; how binding affects PMCA function is unknown. Here, we examined the effects of altered expression of Homer proteins on PMCA-mediated Ca2+ clearance from rat hippocampal neurons in culture. The kinetics of PMCA-mediated recovery from the [Ca2+]i increase evoked by a brief train of action potentials was determined in the soma of single neurons using indo-1-based photometry. Exogenous expression of Homer 1a, Homer 1c or Homer 2a did not affect PMCA function. However, shRNA mediated knockdown of Homer 1 slowed PMCA mediated Ca2+ clearance by 28% relative to cells expressing non-silencing shRNA. The slowed recovery rate in cells expressing Homer 1 shRNA was reversed by expression of a short Homer 2 truncation mutant. These results indicate that constitutively expressed Homer proteins tonically stimulate PMCA function in hippocampal neurons. We propose a model in which binding of short or long Homer proteins to the carboxyl terminus of the PMCA stimulates Ca2+ clearance rate. PMCA-mediated Ca2+ clearance may be stimulated following incorporation of the pump into Homer organized signaling domains and following induction of the Homer 1a immediate early gene.
► We studied the effects of Homer proteins on plasma membrane Ca2+ pump (PMCA) activity.
► shRNA knockdown of Homer 1 slowed PMCA function in hippocampal neurons.
► Over expression of long or short Homer isoforms failed to affect PMCA function.
► A Homer 2 truncation mutant rescued PMCA function in cells expressing Homer 1 shRNA.
► Short and long Homer proteins tonically stimulate PMCA in hippocampal neurons.
Journal: Biochemical and Biophysical Research Communications - Volume 424, Issue 1, 20 July 2012, Pages 76–81