کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1929482 1050460 2012 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Isolation of rat adrenocortical mitochondria
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Isolation of rat adrenocortical mitochondria
چکیده انگلیسی

This report describes a relatively simple and reliable method for isolating adrenocortical mitochondria from rats in good, reasonably pure yield. These organelles, which heretofore have been unobtainable in isolated form from small laboratory animals, are now readily accessible. A high degree of mitochondrial purity is shown by the electron micrographs, as well as the structural integrity of each mitochondrion. That these organelles have retained their functional integrity is shown by their high respiratory control ratios. In general, the biochemical performance of these adrenal cortical mitochondria closely mirrors that of typical hepatic or cardiac mitochondria.


► A method for isolation of adrenocortical mitochondria from the adrenal gland of rats is described.
► The purified isolated mitochondria show excellent morphological integrity.
► The properties of oxidative phosphorylation are excellent.
► The method increases the opportunity of direct analysis of adrenal mitochondria from small animals.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 427, Issue 1, 12 October 2012, Pages 96–99
نویسندگان
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