کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1929538 | 1050462 | 2012 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: DNA methyltransferase inhibitor CDA-II inhibits myogenic differentiation DNA methyltransferase inhibitor CDA-II inhibits myogenic differentiation](/preview/png/1929538.png)
CDA-II (cell differentiation agent II), isolated from healthy human urine, is a DNA methyltransferase inhibitor. Previous studies indicated that CDA-II played important roles in the regulation of cell growth and certain differentiation processes. However, it has not been determined whether CDA-II affects skeletal myogenesis. In this study, we investigated effects of CDA-II treatment on skeletal muscle progenitor cell differentiation, migration and proliferation. We found that CDA-II blocked differentiation of murine myoblasts C2C12 in a dose-dependent manner. CDA-II repressed expression of muscle transcription factors, such as Myogenin and Mef2c, and structural proteins, such as myosin heavy chain (Myh3), light chain (Mylpf) and MCK. Moreover, CDA-II inhibited C1C12 cell migration and proliferation. Thus, our data provide the first evidence that CDA-II inhibits growth and differentiation of muscle progenitor cells, suggesting that the use of CDA-II might affect skeletal muscle functions.
► CDA-II inhibits myogenic differentiation in a dose-dependent manner.
► CDA-II repressed expression of muscle transcription factors and structural proteins.
► CDA-II inhibited proliferation and migration of C2C12 myoblasts.
Journal: Biochemical and Biophysical Research Communications - Volume 422, Issue 3, 8 June 2012, Pages 522–526