کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
192954 | 459756 | 2008 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: A study of the electrochemical oxidation of hydrogen peroxide on a platinum rotating disk electrode in the presence of calcium ions using Michaelis–Menten kinetics and binding isotherm analysis A study of the electrochemical oxidation of hydrogen peroxide on a platinum rotating disk electrode in the presence of calcium ions using Michaelis–Menten kinetics and binding isotherm analysis](/preview/png/192954.png)
The present work demonstrates a potential suppression in the electrochemical signal of H2O2 oxidation due to the presence of Ca2+ ions. A mechanistic scheme was proposed to include a reversible interaction of Ca2+ ions with either the electrode surface binding sites (competitive) or the complex sites (non-competitive). The degree of inhibition was inspected by evaluating the kinetic currents as a function of [Ca2+] applying Koutecky–Levich kinetics. These observations were further supported with models based on enzyme kinetics such as Michaelis–Menten model applying Lineweaver–Burk plot along with non-linear least-square fitting analysis. The experimental results suggests that the strength of the complex binding sites decreases considerably with increasing [Ca2+] and that a single H2O2 molecule is required to combine with one available active binding site.
Journal: Electrochimica Acta - Volume 53, Issue 12, 1 May 2008, Pages 4302–4308