کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1929555 1536782 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Specific phase arrest of cell cycle restores cell viability against tRNA cleavage by killer toxin
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Specific phase arrest of cell cycle restores cell viability against tRNA cleavage by killer toxin
چکیده انگلیسی

Zymocin and PaT are killer toxins that induce cell cycle arrest of sensitive yeast cells in G1 and S phase, respectively. Recent studies have revealed that these two toxins cleave specific tRNAs, indicating that the cell growth impairment is due to the tRNA cleavage. Additionally, we have previously shown that the active domain of colicin D (D-CRD), which also cleaves specific Escherichia coli tRNAs, statically impairs growth when expressed in yeast cells. To verify that phase-specific cell cycle arrest is also induced by the expression of D-CRD, D-CRD and the subunits of zymocin and PaT that have tRNA cleaving activity were expressed in yeast cells and cell cycle status was analyzed. Our results indicate that phase-specific arrest does not commonly occur by tRNA cleavage, and it saves the cell viability. Furthermore, the extent of protein synthesis impairment may determine the phase specificity of cell cycle arrest.


► Cell cycle status of cells expressing each of tRNA-targeting toxins was analyzed.
► Phase specific cell cycle arrest restores the cell viability against tRNA cleavage.
► Remaining translation activity may determine the specificity of cell cycle arrest.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 420, Issue 4, 20 April 2012, Pages 750–754
نویسندگان
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