کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1929755 1050473 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nociceptin is upregulated by FSH signaling in Sertoli cells in murine testes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Nociceptin is upregulated by FSH signaling in Sertoli cells in murine testes
چکیده انگلیسی

In postnatal testes, follicle-stimulating hormone (FSH) acts on somatic Sertoli cells to activate gene expression directly via an intracellular signaling pathway composed of cAMP, cAMP-dependent protein kinase (PKA), and cAMP-response element-binding protein (CREB), and promotes germ cell development indirectly. Yet, the paracrine factors mediating the FSH effects to germ cells remained elusive. Here we show that nociceptin, known as a neuropeptide, is upregulated by FSH through cAMP/PKA/CREB pathway in Sertoli cells in murine testes. Chromatin immunoprecipitation from Sertoli cells shows that CREB phosphorylated at Ser133 associates with prepronociceptin gene encoding nociceptin. Analyses with Sertoli cells and testes demonstrates that both prepronociceptin mRNA and the nociceptin peptide are induced after FSH signaling is activated. In addition, the nociceptin peptide is induced in testes after 9 days post partum following FSH surge. Thus, our findings may identify nociceptin as a novel paracrine mediator of the FSH effects in the regulation of spermatogenesis.


► Nociceptin is traditionally known as a neuropeptide.
► Phosphorylated CREB associates with prepronociceptin gene encoding nociceptin.
► FSH signaling induces prepronociceptin mRNA in Sertoli cells.
► FSH signaling upregulates the nociceptin peptide in Sertoli cells.
► FSH surge follows the nociceptin peptide production in testes after 9 dpp.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 421, Issue 4, 18 May 2012, Pages 678–683
نویسندگان
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