کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1929943 1050481 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Osterix is regulated by Erk1/2 during osteoblast differentiation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Osterix is regulated by Erk1/2 during osteoblast differentiation
چکیده انگلیسی

Osterix (Osx) is a novel zinc finger-containing transcription factor that is essential for osteoblast differentiation and bone formation in bone homeostasis. The mitogen-activated protein (MAP) kinases are a group of evolutionarily conserved proline-directed protein serine/threonine kinases that are activated in response to a variety of extracellular stimuli and mediate signal transduction from the cell surface to the nucleus. Erk1/2 plays essential roles in osteoblast differentiation and in supporting osteoclastogenesis, but the precise molecular signaling mechanisms between Osterix and Erk1/2 are not known. We therefore focused on the relationship between Osterix and Erk1/2 during osteoblast differentiation because BMP signaling induces Erk activation in osteoblasts. We investigated the role of the MAPK pathway in regulating protein levels and transcriptional functions of Osterix. We found that Erk activation by overexpression of constitutively active MEK increased the mRNA and protein levels of Osterix and enhanced the transcriptional activity of Osterix, whereas U0126, an inhibitor of MEK, suppressed the protein levels of Osterix and the transcriptional activity. Also, overexpression of constitutively active MEK stabilized Osterix protein. These results suggest that Erk1/2 regulates a major transcription factor, Osterix, during osteoblast differentiation by increasing its protein stability and transcriptional activity.


► Erk1/2 activation is induced in BMP2-stimulated osteoblast differentiation and increased the protein levels of Osterix.
► Osterix is regulated by Erk1/2 in BMP2-induced osteoblast differentiation.
► Erk1/2 regulates the protein stability of Osterix.
► Erk1/2 regulates the transcriptional and osteogenic activities of Osterix.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 415, Issue 3, 25 November 2011, Pages 472–478
نویسندگان
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