کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1930237 1050493 2012 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The matrix metalloproteinase-7 regulates the extracellular shedding of syndecan-2 from colon cancer cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
The matrix metalloproteinase-7 regulates the extracellular shedding of syndecan-2 from colon cancer cells
چکیده انگلیسی

The cell surface heparan sulfate proteoglycan syndecan-2 regulates the activation of matrix metalloproteinase-7 (MMP-7) as a docking receptor. Here, we demonstrate the role of MMP-7 on syndecan-2 shedding in colon cancer cells. Western blot analysis showed that shed syndecan-2 was found in the culture media from various colon cancer cells. Overexpression of MMP-7 enhanced syndecan-2 shedding, whereas the opposite was true when MMP-7 levels were knocked-down using small inhibitory RNAs. Consistently, HT29 cells treated with MMP-7, but neither MMP-2 nor MMP-9, showed increased shed syndecan-2 in a time- and concentration-dependent manner. Furthermore, MALDI-TOF MS analysis and N-terminal amino acid sequencing revealed that MMP-7 cleaved both recombinant syndecan-2 and an endogenously glycosylated syndecan-2 ectodomain in the N-terminus at Leu149 residue in vitro. Taken together, the data suggest that MMP-7 directly mediates shedding of syndecan-2 from colon cancer cells.


► Overexpression of MMP-7 enhances syndecan-2 shedding.
► MMP-7 enhances the shedding of syndecan-2 from human colon cancer cells.
► MMP-7 cleaved syndecan-2 ectodomain in the N-terminus at Leu149 residue in vitro.
► MMP-7 directly mediates shedding of syndecan-2 from colon cancer cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 417, Issue 4, 27 January 2012, Pages 1260–1264
نویسندگان
, , , , , ,