کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1930413 | 1050510 | 2011 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Discrimination between primary necrosis and apoptosis by necrostatin-1 in Annexin V-positive/propidium iodide-negative cells Discrimination between primary necrosis and apoptosis by necrostatin-1 in Annexin V-positive/propidium iodide-negative cells](/preview/png/1930413.png)
Apoptotic cell death eventually results in secondary necrotic cell death, whereas caspase-independent primary necrotic cell death has been reported and its mechanism involving RIP1 and RIP3 has been recently elucidated. Dual staining with fluorescent Annexin V and propidium iodide (PI) has been used to discriminate apoptotic and necrotic cell death, in which Annexin V-positive/PI-negative staining is regarded as apoptosis and PI-positive staining as necrosis. Here we demonstrate that primary necrotic cells unexpectedly show Annexin V-positive/PI-negative staining before they become PI-positive, and that primary necrotic and apoptotic Annexin V-positive/PI-negative cells can be discriminated by necrostatin-1, an inhibitor of primary necrosis by inhibition of RIP1.
► Annexin V-positive/PI-negative staining is regarded as apoptosis and PI-positive staining as necrosis.
► Primary necrotic cells unexpectedly show Annexin V-positive/PI-negative staining before they become PI-positive.
► Primary necrotic and apoptotic Annexin V-positive/PI-negative cells can be discriminated by necrostatin-1.
Journal: Biochemical and Biophysical Research Communications - Volume 411, Issue 3, 5 August 2011, Pages 569–573