کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1930747 1050526 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Leishmania express a functional Cdc20 homologue
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Leishmania express a functional Cdc20 homologue
چکیده انگلیسی

Our knowledge concerning the mechanisms of cell cycle regulation in organisms belonging to the Trypanosometidae family is limited. Leishmania donovani are parasitic protozoa that cause kala azar, a fatal form of visceral leishmaniasis in humans. Here we provide evidence that the L. donovani genome contains a Cdc20 homologue. Cdc20 is a regulator of the Anaphase Promoting Complex/Cyclosome (APC/C) that mediates ubiquitin-dependent proteasomal degradation of key cell cycle regulators in eukaryotes. We show that L. donovani Cdc20 protein (LdCdc20p) can complement a lack of yeast Cdc20 protein in Saccharomyces cerevisiae cells, validating the functionality of LdCdc20p. Furthermore, we demonstrate cyclic expression of LdCdc20p and that it contains an active RXXL destruction motif, a distinctive feature of proteins targeted for proteasomal degradation by APC/C. Finally, in line with the proteasome mediating LdCdc20p degradation, promastigotes exposed to proteasome inhibitor display elevated LdCdc20p levels. Taken together our data indicate that Leishmania regulate their cell cycle by ubiquitin-dependent proteasomal degradation mediated by the APC/C.


► We have identified in Leishmania a gene encoding for Cdc20 homologue (LdCdc20).
► That complements a lack of yeast Cdc20 protein in S. cerevisiae mutants.
► It contains an active RXXL destruction motif and degraded by proteasome.
► That is responsible for cyclic expression of this protein.
► LdCdc20 overexpression attenuates cell cycle at G1.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 408, Issue 1, 29 April 2011, Pages 71–77
نویسندگان
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