A role for tumor protein TPD52 phosphorylation in endo-membrane trafficking during cytokinesis

► D52 localizes to vesicles at the mid-line in multinucleated cells. ► Expression of a D52 serine136/alanine mutant induced multinucleation of cells. ► D52 localizes to VAMP 8 positive endosomes necessary for cytokinesis. ► The Ca2+-dependent phosphorylation of D52 regulates cytokinesis.