کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1931105 | 1536786 | 2010 | 5 صفحه PDF | دانلود رایگان |
Cysteamine (1) was reported many years ago to reversibly inhibit lentil seedling amine oxidase, through the formation of a complex with thioacetaldehyde, the turnover product of 1. Herein, cysteamine (1) and its analogs 2-(methylamino)ethanethiol (3) and 3-aminopropanethiol (6) were found to be reversible inhibitors of bovine plasma amine oxidase (BPAO), but 2-(methylthio)ethylamine (7) was determined to be a weak irreversible inhibitor of BPAO. Based on our results, indicating the necessity of a sulfhydryl-amine for reversible inactivation of BPAO, the failure of inhibited BPAO to recover activity after gel filtration, the first-order kinetics of activity recovery upon dialysis, and 2,4,6-trihydroxyphenylalanine quinine (TPQ) cofactor transformation which indicated from the results of phenylhydrazine titration and substrate protection, we propose a mechanism for the reversible inactivation of BPAO by 1 involving the formation of a cofactor adduct, thiazolidine, between BPAO and 1.
Research highlights
► Cysteamine (1) and its analogs, 2-(methylamino)ethanethiol (3) and 3-aminopropanethiol (6), were found to be reversible inhibitors of bovine plasma amine oxidase (BPAO), but 2-(methylthio)ethylamine (7) was a weak irreversible inhibitor of BPAO.
► Sulfhydryl-amine was necessary for reversible inactivation of BPAO.
► The reversible inactivation mechanism by 1 is proposed that BPAO may be inactivated by forming a cofactor adduct, thiazolidine, with 1.
Journal: Biochemical and Biophysical Research Communications - Volume 403, Issues 3–4, 17 December 2010, Pages 442–446