کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1931147 1050542 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Changes in cytosolic glucose level in ATP stimulated live astrocytes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Changes in cytosolic glucose level in ATP stimulated live astrocytes
چکیده انگلیسی

Astrocytes which lie between brain capillaries and neuronal terminals are the primary site of glucose uptake and have a key role in coupling synaptic activity to glucose utilization in the central nervous system (CNS). We used a fluorescence resonance energy transfer (FRET) based approach to monitor cytosolic glucose in astrocytes. We determined the effect of increasing extracellular glucose concentrations on FRET ratio as a measure of increased cytosolic glucose in astrocytes. By briefly raising extracellular glucose concentration, astrocytes responded promptly by increased cytosolic glucose levels, which was manifested by decreased time-dependent FRET ratio. The FRET ratio fall-time recorded at low extracellular d-glucose concentration change (from 0 to 0.5 mM) was 53 s, whereas 17 s was recorded by raising extracellular concentration of d-glucose from 0 to 10 mM, which is likely due to facilitated d-glucose entry along the increased d-glucose gradient across the plasmalemma. The relationship between the extracellular glucose concentration and the FRET ratio change is limited to the maximal ratio change, where the d-glucose plasma membrane permeability is balanced by the cytosolic utilization. We measured the effect of extracellular ATP, an important extracellular messenger for astrocyte-to-astrocyte communication, on intracellular glucose concentration. The results show that stimulation of astrocytes with ATP (1 mM) decreases cytosolic glucose concentration with a time constant of ∼145 s. The mechanism of this change is discussed.

Research highlights
► The extracellular glucose concentration affects cytosolic glucose in astrocytes.
► Raising extracellular glucose to 10 mM increased cytosolic glucose levels in 17 s.
► The stimulation of astrocytes with ATP decreases cytosolic glucose concentration.
► The time constant of ATP induced glucose decrease was 145 s.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 405, Issue 2, 11 February 2011, Pages 308–313
نویسندگان
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