کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1931200 1050544 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A sensitive, simple assay of mitochondrial ATP synthesis of cultured mammalian cells suitable for high-throughput analysis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
A sensitive, simple assay of mitochondrial ATP synthesis of cultured mammalian cells suitable for high-throughput analysis
چکیده انگلیسی

A new assay has been developed to measure mitochondrial ATP synthesis of cultured mammalian cells. Cells in a microplate are exposed to streptolysin O to make plasma membranes permeable without damaging mitochondrial function and ATP synthesis is monitored by luciferase. Addition of inhibitors of FoF1-ATP synthase (FoF1), respiratory chain, TCA cycle and ATP/ADP translocator, as well as knockdown of β-subunit of FoF1, resulted in loss of ATP synthesis. Compared with the conventional procedures that need mitochondria fractionation and detergent, this assay is simple, sensitive and suitable for high-throughput analysis of genes and drugs that could affect mitochondrial functional integrity as represented by ATP synthesis activity.

Research highlights
► A sensitive, simple assay for mitochondrial ATP synthesis that represents mitochondrial functional integrity.
► It needs no detergent and no mitochondrial fractionation.
► Good for high-throughput analysis of mammalian cells cultured on a microplate.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 401, Issue 4, 29 October 2010, Pages 538–543
نویسندگان
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