کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1931433 1050552 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Assessment of chronological lifespan dependent molecular damages in yeast lacking mitochondrial antioxidant genes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Assessment of chronological lifespan dependent molecular damages in yeast lacking mitochondrial antioxidant genes
چکیده انگلیسی

The free radical theory of aging states that oxidative damage to biomolecules causes aging and that antioxidants neutralize free radicals and thus decelerate aging. Mitochondria produce most of the reactive oxygen species, but at the same time have many antioxidant enzymes providing protection from these oxidants. Expecting that cells without mitochondrial antioxidant genes would accumulate higher levels of oxidative damage and, therefore, will have a shorter lifespan, we analyzed oxidative damages to biomolecules in young and chronologically aged mutants lacking the mitochondrial antioxidant genes: GRX2, CCP1, SOD1, GLO4, TRR2, TRX3, CCS1, SOD2, GRX5, and PRX1. Among these mutants, ccp1Δ, trx3Δ, grx5Δ, prx1Δ, mutants were sensitive to diamide, and ccs1Δ and sod2Δ were sensitive to both diamide and menadione. Most of the mutants were less viable in stationary phase. Chronologically aged cells produced higher amount of superoxide radical and accumulated higher levels of oxidative damages. Even though our results support the findings that old cells harbor higher amount of molecular damages, no significant difference was observed between wild type and mutant cells in terms of their damage content.

Research highlights
► Oxidative molecular damages accumulate as cells age.
► Absence of CCS1 gene leads to shorter lifespan and higher oxidative damages to biomolecules.
► Deficiency in mitochondrial antioxidant genes cause shorter lifespan.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 400, Issue 1, 10 September 2010, Pages 106–110
نویسندگان
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