کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1931440 | 1050552 | 2010 | 6 صفحه PDF | دانلود رایگان |
Essential for the normal functioning of a cell is the maintenance of genomic integrity. Failure in this process is often catastrophic for the organism, leading to cell death or mis-proliferation. Central to genomic integrity is the faithful replication of DNA during S phase. The GINS complex has recently come to light as a critical player in DNA replication through stabilization of MCM2–7 and Cdc45 as a member of the CMG complex which is likely responsible for the processivity of helicase activity during S phase. The GINS complex is made up of 4 members in a 1:1:1:1 ratio: Psf1, Psf2, Psf3, And Sld5. Here we present the first analysis of the function of the Sld5 subunit in a multicellular organism. We show that Drosophila Sld5 interacts with Psf1, Psf2, and Mcm10 and that mutations in Sld5 lead to M and S phase delays with chromosomes exhibiting hallmarks of genomic instability.
Research highlights
► Drosophila Sld5 interacts with Psf1, PPsf2, and Mcm10.
► Haploinsufficiency of Sld5 leads to M-phase delay and genomic instability.
► Sld5 is also required for normal S phase progression.
Journal: Biochemical and Biophysical Research Communications - Volume 400, Issue 1, 10 September 2010, Pages 145–150