کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1931477 1050554 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Vinculin activates inside-out signaling of integrin αIIbβ3 in Chinese hamster ovary cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Vinculin activates inside-out signaling of integrin αIIbβ3 in Chinese hamster ovary cells
چکیده انگلیسی

Although vinculin is used frequently as a marker for integrin-mediated focal adhesion complexes, how it regulates the activation of integrin is mostly unknown. In this study, we examined whether vinculin would activate integrin in Chinese hamster ovary (CHO) cells expressing human integrin αIIbβ3. Silencing of vinculin by lentiviral transduction with a short hairpin RNA sequence affected the binding of PAC-1 (an antibody recognizing activated human αIIbβ3) to a constitutively active form of αIIbβ3 (α6Bβ3) expressed on CHO cells, while its inhibitory effects were much weaker than those of talin-1. Overexpression of an active form of vinculin without intramolecular interactions, but not the full length one, induced PAC-1 binding to native αIIbβ3 expressed on CHO cells in a manner dependent on talin-1. On the other hand, silencing of talin-1, but not vinculin, failed to induce cell spreading of α6Bβ3-CHO cells on fibrinogen, even in the presence of PT 25-2, a monoclonal antibody that activates αIIbβ3. Thus, an active form of vinculin could induce αIIbβ3 inside-out signaling through the actions of talin-1, while vinculin was dispensable for outside-in signaling.

Research highlights
► Overexpression of an active form of vinculin increases ligand binding to integrin αIIbβ3.
► Vinculin-induced activation of αIIbβ3 depends on the action of talin-1.
► Vinculin is not required for outside-in signaling.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 400, Issue 3, 24 September 2010, Pages 323–328
نویسندگان
, , , , , , , ,