کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1931590 1050557 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Breast cancer cells attract the migration of adipose tissue-derived stem cells via the PDGF-BB/PDGFR-β signaling pathway
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Breast cancer cells attract the migration of adipose tissue-derived stem cells via the PDGF-BB/PDGFR-β signaling pathway
چکیده انگلیسی

The origin of vascular cells in tumors is unknown, but it is believed that tumors use cells from the host to build new vessels. To determine whether adipose tissue stem cells (ASCs) could be attracted by cancer cells, we performed migration assays in which ASCs were seeded on a transwell migration system top chamber and tumor-conditioned medium was placed in the bottom chamber. Our data showed that a significant number of ASCs migrated toward the tumor-conditioned medium (p < 0.0001), and migration of human ASCs significantly (p < 0.0001) increased in response to increased concentrations of recombinant PDGF-BB. In addition, neutralizing antibodies to PDGF receptor (PDGFR)-β decreased migration of ASCs toward a breast cancer-conditioned medium to the level of serum-free control. These data suggest that tumor cell-derived PDGF-BB is an important factor in governing the microenvironment interaction between tumor cells and local tissue-resident stem cells.

Research highlights
► Adipose tissue-derived stem cells (ASC) migrated toward the tumor-conditioned medium.
► Migration of ASCs increased in response to increased concentrations of PDGF-BB.
► Neutralizing antibodies to PDGFR-β decreased migration of ASCs.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 398, Issue 3, 30 July 2010, Pages 601–605
نویسندگان
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