کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1931611 | 1050558 | 2010 | 6 صفحه PDF | دانلود رایگان |
Tensins are proposed cytoskeleton-regulating proteins. However, Tensin2 additionally inhibits Akt signalling and cell survival. Structural modelling of the Tensin2 phosphatase (PTPase) domain revealed an active site-like pocket receptive towards phosphoinositides. Tensin2-expressing HEK293 cells displayed negligible levels of plasma membrane phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P3) under confocal microscopy. However, mock-transfected cells, and Tensin2 cells harbouring a putative phosphatase-inactivating mutation, exhibited significant PtdIns(3,4,5)P3 levels, which decreased upon phosphatidylinositol 3-kinase inhibition with LY294002. In contrast, wtTensin3, mock and mutant cells were identical in membrane PtdIns(3,4,5)P3 and Akt phosphorylation. In vitro lipid PTPase activity was however undetectable in isolated recombinant PTPase domains of both Tensins, indicating a possible loss of structural stability when expressed in isolation. In summary, we provide evidence that Tensin2, in addition to regulating cytoskeletal dynamics, influences phosphoinositide-Akt signalling through its PTPase domain.
Research highlights
► Tensin2 phosphatase domain is structurally receptive for phosphoinositide interaction.
► Tensin2 overexpression causes reduction of plasma membrane PtdIns(3,4,5)P3 in cells.
► A phosphatase-inactivating mutation in Tensin2 reverses this effect.
► In contrast, Tensin3 overexpression has no effect on plasma membrane PtdIns(3,4,5)P3.
► Tensin2 but not Tensin3 reduces levels of intracellular Akt phosphorylation.
Journal: Biochemical and Biophysical Research Communications - Volume 399, Issue 3, 27 August 2010, Pages 396–401