Dynamics of lipid droplets induced by the hepatitis C virus core protein

► Hepatitis C virus uses lipid droplets (LD) onto which HCV core proteins bind. ► HCV core proteins on LDs facilitate viral particle assembly. ► We used a novel combination of CARS, two-photon fluorescence, and DIC microscopies. ► Particle tracking experiments show that core slowly affects LD localization. ► Particle tracking measured the change in speed and directionality of LD movement.