کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1931680 1050560 2010 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Novel protein interactors of urokinase-type plasminogen activator receptor
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Novel protein interactors of urokinase-type plasminogen activator receptor
چکیده انگلیسی

The urokinase-type plasminogen activator receptor (uPAR) has been implicated in tumor growth and metastasis. The crystal structure of uPAR revealed that the external surface is largely free to interact with a number of proteins. Additionally, due to absence of an intracellular cytoplasmic protein domain, many of the biological functions of uPAR necessitate interactions with other proteins. Here, we used yeast two-hybrid screening of breast cancer cDNA library to identify hSpry1 and HAX1 proteins as putative candidate proteins that interact with uPAR bait constructs. Interaction between these two candidates and uPAR was confirmed by GST-pull down, co-immunoprecipitation assays and confocal microscopy. These novel interactions that have been identified may also provide further evidence that uPAR can interact with a number of other proteins which may influence a range of biological functions.

Research highlights
► Screening of breast cancer cDNA library identified novel uPAR candidate proteins.
► hSpry1 interaction with uPAR has been identified and confirmed.
► Novel interaction between uPAR and HAX1 has also been recognized.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 399, Issue 4, 3 September 2010, Pages 738–743
نویسندگان
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