کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1932044 1050572 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effective knockdown of multiple target genes by expressing the single transcript harbouring multi-cistronic shRNAs
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Effective knockdown of multiple target genes by expressing the single transcript harbouring multi-cistronic shRNAs
چکیده انگلیسی

Gene silencing by RNA interference (RNAi) using short interfering RNAs (siRNAs) or short hairpin RNAs (shRNAs) has become a valuable tool for evaluating the target gene function. Here, we report an approach for silencing multiple target genes simultaneously by expressing one single transcript encoding different target shRNAs. We first constructed the cytomegalovirus (CMV) promoter-driven expression vectors, each of which expresses microRNA mir-30-mimicked shRNA specifically targeting X-chromosome-linked inhibitor of apoptosis protein (XIAP), Akt, or Bcl-2. Adenovirus harbouring each shRNA expression cassette silenced corresponding target gene expression. Using these mono-cistronic shRNA cassettes, we again constructed the CMV promoter-driven expression vector, into which multi-cistronic shRNAs for XIAP, Akt and Bcl-2 in order were cloned. Adenovirus delivering this multi-cistronic expression cassette silenced each of the target genes as effectively as adenovirus containing individual shRNA did. Our data indicate that single promoter-driven multi-cistronic shRNAs effectively silence multiple target genes. Our approach provides a new smart tool for silencing multiple target genes and will potentially serve as an RNAi-based tailored therapy requiring suppression of target gene expression.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 396, Issue 4, 11 June 2010, Pages 861–865
نویسندگان
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